Description
The GeneJET Plasmid Maxiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. Quantity: 10 preps.
The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,
in vitro transcription, and transfection of robust cell lines.
Highlights - Efficient - high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
- High concentration of DNA purified - > 400 ng/µL
•Convenient - spin column format, centrifuge or vacuum protocols - Fast - procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications- Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
- Transfection of robust cell lines
- FastDigest restriction enzyme digestion
- Automated fluorescent sequencing
- PCR
- Cloning
- Transformation
- In vitro transcription
Includes- Resuspension Solution
- Lysis Solution
- Neutralization Solution
- Endotoxin Binding Reagent
- Wash Solution I (concentrated)
- Wash Solution II (concentrated)
- RNase A Solution
- Elution Buffer (10 mM Tris-HCl, pH 8.5)
- GeneJET Maxi Purification Columns pre-assembled with collection tubes (50 mL)
- Collection tubes (50 mL)
Hazardous: No
Quality Control:
- The GeneJET Plasmid Maxiprep Kit is qualified by isolating high copy number plasmid DNA from 250 mL of overnight E. coli culture (OD600 = 2) grown in LB medium following the protocol outlined in the manual.
- The kit passes QC requirements if the purified plasmid DNA has an A260/280 ratio between 1.8 and 1.9 and a dominant band of supercoiled plasmid DNA is observed after agarose gel electrophoresis.
- The functional quality of the plasmid DNA is evaluated by digestion with restriction endonucleases.
Storage Condition: Ambient Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
- Transfection of robust cell lines
- FastDigest restriction enzyme digestion
- Automated fluorescent sequencing
- PCR
- Cloning
- Transformation
- In vitro transcription